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ARM Megatests

ARM Megatests #336

GitHub Actions / JUnit Test Report failed Apr 11, 2025 in 0s

2 tests run, 0 passed, 0 skipped, 2 failed.

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Check failure on line 1 in Test pipeline with a minimum mapped read threshold of 90

See this annotation in the file changed.

@github-actions github-actions / JUnit Test Report

Test pipeline with a minimum mapped read threshold of 90.Params: --min_mapped_reads 90

Assertion failed: 

1 of 2 assertions failed
Raw output
Nextflow stdout:

N E X T F L O W  ~  version 25.02.3-edge
Launching `/home/runner/work/rnaseq/rnaseq/tests/../main.nf` [confident_darwin] DSL2 - revision: 85c9b75b8b
Downloading plugin [email protected]

------------------------------------------------------
                                        ,--./,-.
        ___     __   __   __   ___     /,-._.--~'
  |\ | |__  __ /  ` /  \ |__) |__         }  {
  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                        `._,._,'
  nf-core/rnaseq 3.19.0dev
------------------------------------------------------
Input/output options
  input                                              : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/samplesheet/v3.10/samplesheet_test.csv
  outdir                                             : /home/runner/work/rnaseq/rnaseq/~/tests/716928385ff6315bd23e989a7cf7e4a1/output

Reference genome options
  fasta                                              : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genome.fasta
  gtf                                                : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes_with_empty_tid.gtf.gz
  gff                                                : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes.gff.gz
  transcript_fasta                                   : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/transcriptome.fasta
  additional_fasta                                   : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/gfp.fa.gz
  hisat2_index                                       : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/hisat2.tar.gz
  rsem_index                                         : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/rsem.tar.gz
  salmon_index                                       : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/salmon.tar.gz
  hisat2_build_memory                                : 3.GB

Read filtering options
  bbsplit_fasta_list                                 : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/bbsplit_fasta_list.txt

UMI options
  umitools_bc_pattern                                : NNNN

Alignment options
  pseudo_aligner                                     : salmon
  min_mapped_reads                                   : 90

Process skipping options
  skip_bbsplit                                       : false

Institutional config options
  config_profile_name                                : Test profile
  config_profile_description                         : Minimal test dataset to check pipeline function

Generic options
  pipelines_testdata_base_path                       : s3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/
  trace_report_suffix                                : 2025-04-11_19-51-30

Core Nextflow options
  runName                                            : confident_darwin
  containerEngine                                    : docker
  container.BBMAP_BBSPLIT                            : community.wave.seqera.io/library/bbmap:39.10--168578230ff6490a
  container.BEDTOOLS_GENOMECOV                       : community.wave.seqera.io/library/bedtools:2.31.1--7c4ce4cb07c09ee4
  container.BRACKEN_BRACKEN                          : community.wave.seqera.io/library/bracken:2.9--3d5af74ed6966fc1
  container.CAT_FASTQ                                : community.wave.seqera.io/library/coreutils:9.5--ae99c88a9b28c264
  container.CUSTOM_CATADDITIONALFASTA                : community.wave.seqera.io/library/python:3.12.2--8aba3d9ac529ff19
  container.CUSTOM_GETCHROMSIZES                     : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.CUSTOM_TX2GENE                           : community.wave.seqera.io/library/python:3.10.4--a643e28963f01eef
  container.DESEQ2_QC                                : community.wave.seqera.io/library/r-base_r-optparse_r-ggplot2_r-rcolorbrewer_pruned:d06fadc02f8422a2
  container.DUPRADAR                                 : community.wave.seqera.io/library/bioconductor-dupradar:1.32.0--dcb3a3be56be6612
  container.FASTP                                    : community.wave.seqera.io/library/fastp:0.23.4--f8cefc1e5f7a782e
  container.FASTQC                                   : community.wave.seqera.io/library/fastqc:0.12.1--af7a5314d5015c29
  container.FQ_LINT                                  : community.wave.seqera.io/library/fq:0.12.0--45ea0ea1c4d20daa
  container.FQ_SUBSAMPLE                             : community.wave.seqera.io/library/fq:0.12.0--45ea0ea1c4d20daa
  container.GFFREAD                                  : community.wave.seqera.io/library/gffread:0.12.7--33b95f1cfcc0e572
  container.GTF2BED                                  : community.wave.seqera.io/library/perl:5.26.2--e6570c5f08d3e12c
  container.GTF_FILTER                               : community.wave.seqera.io/library/python:3.9.5--0ee01a3faebf676a
  container.GUNZIP                                   : community.wave.seqera.io/library/grep_sed_tar:4f61255d5eff93dc
  container.HISAT2_ALIGN                             : community.wave.seqera.io/library/hisat2_samtools:9ef5e68250d2aa60
  container.HISAT2_BUILD                             : community.wave.seqera.io/library/hisat2:2.2.1--df34d2bb25ac6de5
  container.HISAT2_EXTRACTSPLICESITES                : community.wave.seqera.io/library/hisat2:2.2.1--df34d2bb25ac6de5
  container.KALLISTO_INDEX                           : community.wave.seqera.io/library/kallisto:0.51.1--b63691b6841c7a52
  container.KALLISTO_QUANT                           : community.wave.seqera.io/library/kallisto:0.51.1--b63691b6841c7a52
  container.KRAKEN2_KRAKEN2                          : community.wave.seqera.io/library/kraken2_coreutils_pigz:0892e153c7954635
  container.MULTIQC                                  : community.wave.seqera.io/library/multiqc:1.27--b0d1ffb40dfd9e97
  container.MULTIQC_CUSTOM_BIOTYPE                   : community.wave.seqera.io/library/python:3.9.5--0ee01a3faebf676a
  container.PICARD_MARKDUPLICATES                    : community.wave.seqera.io/library/picard:3.1.1--bae6cd265e6bf3d3
  container.PREPROCESS_TRANSCRIPTS_FASTA_GENCODE     : community.wave.seqera.io/library/sed:4.7--c9d1b71422302845
  container.PRESEQ_LCEXTRAP                          : community.wave.seqera.io/library/preseq:3.2.0--2789d8b704b33613
  container.QUALIMAP_RNASEQ                          : community.wave.seqera.io/library/qualimap:2.3--c1797c2253925b3a
  container.RSEM_CALCULATEEXPRESSION                 : community.wave.seqera.io/library/rsem_star:5acb4e8c03239c32
  container.RSEM_MERGE_COUNTS                        : community.wave.seqera.io/library/sed:4.7--c9d1b71422302845
  container.RSEM_PREPAREREFERENCE                    : community.wave.seqera.io/library/rsem_star:5acb4e8c03239c32
  container.RSEQC_BAMSTAT                            : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_INFEREXPERIMENT                    : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_INNERDISTANCE                      : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_JUNCTIONANNOTATION                 : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_JUNCTIONSATURATION                 : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_READDISTRIBUTION                   : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_READDUPLICATION                    : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_TIN                                : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.SALMON_INDEX                             : community.wave.seqera.io/library/salmon:1.10.3--fcd0755dd8abb423
  container.SALMON_QUANT                             : community.wave.seqera.io/library/salmon:1.10.3--fcd0755dd8abb423
  container.SAMTOOLS_FLAGSTAT                        : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_IDXSTATS                        : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_INDEX                           : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_SORT                            : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_STATS                           : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SORTMERNA                                : community.wave.seqera.io/library/sortmerna:4.3.7--6502243397c065ba
  container.STAR_ALIGN                               : community.wave.seqera.io/library/htslib_samtools_star_gawk:311d422a50e6d829
  container.STAR_ALIGN_IGENOMES                      : community.wave.seqera.io/library/star_samtools_gawk:bef5708b1c54889a
  container.STAR_GENOMEGENERATE                      : community.wave.seqera.io/library/htslib_samtools_star_gawk:311d422a50e6d829
  container.STAR_GENOMEGENERATE_IGENOMES             : community.wave.seqera.io/library/star_samtools_gawk:bef5708b1c54889a
  container.STRINGTIE_STRINGTIE                      : community.wave.seqera.io/library/stringtie:2.2.3--3c30041821e5a79a
  container.SUBREAD_FEATURECOUNTS                    : community.wave.seqera.io/library/subread:2.0.6--1968a7457b0ed34e
  container.SUMMARIZEDEXPERIMENT_SUMMARIZEDEXPERIMENT: community.wave.seqera.io/library/bioconductor-summarizedexperiment:1.32.0--13051b66560c2065
  container.TRIMGALORE                               : community.wave.seqera.io/library/cutadapt_trim-galore_pigz:a98edd405b34582d
  container.TXIMETA_TXIMPORT                         : community.wave.seqera.io/library/bioconductor-tximeta:1.20.1--cd025af46d4ef23a
  container.UCSC_BEDCLIP                             : community.wave.seqera.io/library/ucsc-bedclip:377--41de764ea1c26af4
  container.UCSC_BEDGRAPHTOBIGWIG                    : community.wave.seqera.io/library/ucsc-bedgraphtobigwig:469--f66c00635e28f216
  container.UMICOLLAPSE                              : community.wave.seqera.io/library/umicollapse:1.1.0--74864b350c72f3a0
  container.UMITOOLS_DEDUP                           : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UMITOOLS_EXTRACT                         : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UMITOOLS_PREPAREFORRSEM                  : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UNTAR                                    : community.wave.seqera.io/library/grep_sed_tar:4f61255d5eff93dc
  launchDir                                          : /home/runner/work/rnaseq/rnaseq/~/tests/716928385ff6315bd23e989a7cf7e4a1
  workDir                                            : /home/runner/work/rnaseq/rnaseq/~/tests/716928385ff6315bd23e989a7cf7e4a1/work
  projectDir                                         : /home/runner/work/rnaseq/rnaseq
  userName                                           : runner
  profile                                            : test,docker
  configFiles                                        : /home/runner/work/rnaseq/rnaseq/nextflow.config, /home/runner/work/rnaseq/rnaseq/nextflow.config, /home/runner/work/rnaseq/rnaseq/tests/nextflow.config

!! Only displaying parameters that differ from the pipeline defaults !!
------------------------------------------------------
* The pipeline
    https://doi.org/10.5281/zenodo.1400710

* The nf-core framework
    https://doi.org/10.1038/s41587-020-0439-x

* Software dependencies
    https://github.com/nf-core/rnaseq/blob/master/CITATIONS.md

WARN: The following invalid input values have been detected:

* --modules_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/modules/


WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  Both '--gtf' and '--gff' parameters have been provided.
  Using GTF file as priority.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  '--transcript_fasta' parameter has been provided.
  Make sure transcript names in this file match those in the GFF/GTF file.

  Please see:
  https://github.com/nf-core/rnaseq/issues/753
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
WARN: The operator `first` is useless when applied to a value channel which returns a single value by definition
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[b6/59e7e3] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:UNTAR_SALMON_INDEX (salmon.tar.gz)
[86/9b9004] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz)
[40/043ffe] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz)
[2e/4f347a] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_IAA_30M_REP1)
[3e/5fd0a2] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_UNINDUCED_REP1)
[36/5b454c] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ (WT_REP1)
[7a/899825] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ (RAP1_UNINDUCED_REP2)
[50/9b1e41] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (WT_REP2)
[5d/b97e4a] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER (genome.fasta)
[ab/542a95] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_UNINDUCED_REP1)
[5c/329b68] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_UNINDUCED_REP1)
[87/f150ef] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_IAA_30M_REP1)
[3b/bd7548] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_IAA_30M_REP1)
[05/bf0f30] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_UNINDUCED_REP2)
[50/745694] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (WT_REP1)
[bf/1bac23] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP2)
[46/10c088] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP2)
[21/c862dc] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA (null)
[6a/0d8b5c] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (RAP1_UNINDUCED_REP1)
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[ea/2ed0ad] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_UNINDUCED_REP2)
[ad/603482] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_UNINDUCED_REP2)
[24/e9b48c] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP1)
[8c/166b3e] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP1)
[e4/15cd86] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (RAP1_IAA_30M_REP1)
[81/7096c0] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (WT_REP2)
[43/b01c9a] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED (genome_gfp.gtf)
[a6/110b43] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome_gfp.fasta)
[69/801550] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:STAR_GENOMEGENERATE (genome_gfp.fasta)
[ef/4cf607] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT (null)
[f4/342fcb] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (RAP1_UNINDUCED_REP2)
[55/0b931b] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (WT_REP1)
[cd/d6b2ca] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (WT_REP2)
[97/040be2] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (RAP1_IAA_30M_REP1)
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[d1/1f6916] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (RAP1_UNINDUCED_REP1)
[51/5aef71] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (RAP1_UNINDUCED_REP2)
[32/ef0fe7] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (WT_REP1)
[8a/309098] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (WT_REP2)
[27/00d2c0] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (RAP1_IAA_30M_REP1)
[eb/5a3876] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (RAP1_UNINDUCED_REP1)
[36/4375f6] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (RAP1_UNINDUCED_REP2)
[4e/4b626a] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP2)
[2f/5fb62d] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (WT_REP2)
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[20/2baa6e] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (RAP1_IAA_30M_REP1)
[f2/e959b1] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_IAA_30M_REP1)
[cf/3c5c97] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_UNINDUCED_REP1)
[71/92aba6] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (RAP1_UNINDUCED_REP1)
[22/c124a1] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_UNINDUCED_REP2)
[56/332c88] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (RAP1_UNINDUCED_REP2)
[87/711de9] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (WT_REP1)
[91/09a4f1] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (WT_REP2)
[70/baf0d6] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (WT_REP2)
[f9/3e35cf] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (RAP1_UNINDUCED_REP1)
[f9/24aea1] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (RAP1_UNINDUCED_REP1)
[df/0d3e2f] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (RAP1_UNINDUCED_REP2)
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[bf/64ef19] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (RAP1_UNINDUCED_REP2)
[71/5d3ea7] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:FQ_SUBSAMPLE (WT_REP1)
[50/6ac50b] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (WT_REP2)
[bc/0685d6] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (RAP1_UNINDUCED_REP1)
[0b/a2c6eb] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES (RAP1_UNINDUCED_REP1)
[a0/1539c8] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (RAP1_UNINDUCED_REP2)
[e3/ae09af] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES (RAP1_UNINDUCED_REP2)
[9a/22430c] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:SALMON_QUANT (WT_REP1)
[d7/698fd1] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (RAP1_IAA_30M_REP1)
[1a/d6885a] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (RAP1_IAA_30M_REP1)
[8b/7e3c20] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX (RAP1_UNINDUCED_REP1)
[4b/5a76c4] Submitted process > NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ (RAP1_UNINDUCED_REP1)
[49/a2fb4e] Submitted process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS (RAP1_UNINDUCED_REP1)
[7f/03b044] Submitted process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE (RAP1_UNINDUCED_REP1)
[22/ecda65] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_FW (RAP1_UNINDUCED_REP1)
[b4/defc4b] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_REV (RAP1_UNINDUCED_REP1)
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[ed/963aeb] Submitted process > NFCORE_RNASEQ:RNASEQ:DUPRADAR (RAP1_UNINDUCED_REP1)
[b4/19a2ca] Submitted process > NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ (RAP1_UNINDUCED_REP2)
[63/46fdb6] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX (RAP1_UNINDUCED_REP2)
[66/14dcea] Submitted process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE (RAP1_UNINDUCED_REP2)
[3e/a1bc0e] Submitted process > NFCORE_RNASEQ:RNASEQ:DUPRADAR (RAP1_UNINDUCED_REP2)
[21/6f3a88] Submitted process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS (RAP1_UNINDUCED_REP2)
[7b/fb9a59] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_FW (RAP1_UNINDUCED_REP2)
[05/9c3923] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_REV (RAP1_UNINDUCED_REP2)
[26/e7b4f0] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (RAP1_IAA_30M_REP1)
[8f/8fea42] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES (RAP1_IAA_30M_REP1)
[c7/260f3e] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (WT_REP1)
[68/13ed6e] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)
[6c/a17d20] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_BAMSTAT (RAP1_UNINDUCED_REP1)
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[53/33118d] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDISTRIBUTION (RAP1_UNINDUCED_REP1)
[73/0541cc] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INNERDISTANCE (RAP1_UNINDUCED_REP1)
[59/56a19c] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONANNOTATION (RAP1_UNINDUCED_REP1)
[a1/3b8719] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONSATURATION (RAP1_UNINDUCED_REP1)
[9c/e37dee] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INFEREXPERIMENT (RAP1_UNINDUCED_REP1)
[07/bc89ea] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDUPLICATION (RAP1_UNINDUCED_REP1)
[ee/432675] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE (RAP1_UNINDUCED_REP1)
[52/1522f9] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDCLIP (RAP1_UNINDUCED_REP1)
[e1/b55365] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDCLIP (RAP1_UNINDUCED_REP1)
[37/fcbc88] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDUPLICATION (RAP1_UNINDUCED_REP2)
[10/c24a93] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDISTRIBUTION (RAP1_UNINDUCED_REP2)
[c5/a1b834] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_BAMSTAT (RAP1_UNINDUCED_REP2)
[96/81489b] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONANNOTATION (RAP1_UNINDUCED_REP2)
[05/16f6ae] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONSATURATION (RAP1_UNINDUCED_REP2)
[06/fa7964] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INNERDISTANCE (RAP1_UNINDUCED_REP2)
[05/1ca0b6] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INFEREXPERIMENT (RAP1_UNINDUCED_REP2)
[a2/d3e726] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE (RAP1_UNINDUCED_REP2)
[56/1ca58d] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDCLIP (RAP1_UNINDUCED_REP2)
[ec/93bf33] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDCLIP (RAP1_UNINDUCED_REP2)
[4a/71149e] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:CUSTOM_TX2GENE (null)
[30/0f77dd] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_REV (RAP1_IAA_30M_REP1)
[e4/afc0b9] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_FW (RAP1_IAA_30M_REP1)
[1e/94f75d] Submitted process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE (RAP1_IAA_30M_REP1)
[19/f34ca0] Submitted process > NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ (RAP1_IAA_30M_REP1)
[e6/72147e] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX (RAP1_IAA_30M_REP1)
[75/f46f7a] Submitted process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS (RAP1_IAA_30M_REP1)
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[f8/c58c99] Submitted process > NFCORE_RNASEQ:RNASEQ:DUPRADAR (RAP1_IAA_30M_REP1)
[5c/2b5f2a] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG (RAP1_UNINDUCED_REP1)
[8d/855511] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG (RAP1_UNINDUCED_REP1)
[c4/71b881] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG (RAP1_UNINDUCED_REP2)
[42/f0beac] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG (RAP1_UNINDUCED_REP2)
[01/bbc1cd] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:TXIMETA_TXIMPORT
[ed/1080f2] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDCLIP (RAP1_IAA_30M_REP1)
[e8/e826b2] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDCLIP (RAP1_IAA_30M_REP1)
[1b/1a7787] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDUPLICATION (RAP1_IAA_30M_REP1)
[12/01dfec] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDISTRIBUTION (RAP1_IAA_30M_REP1)
[5a/64e04f] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONSATURATION (RAP1_IAA_30M_REP1)
[75/316af4] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONANNOTATION (RAP1_IAA_30M_REP1)
[b0/d84773] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INFEREXPERIMENT (RAP1_IAA_30M_REP1)
[dd/30d5da] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INNERDISTANCE (RAP1_IAA_30M_REP1)
[83/0705b9] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_BAMSTAT (RAP1_IAA_30M_REP1)
[72/70e1de] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE (RAP1_IAA_30M_REP1)
[d6/8f2ff9] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (WT_REP1)
[90/8eee47] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (WT_REP1)
[27/040e86] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG (RAP1_IAA_30M_REP1)
[64/84bca6] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG (RAP1_IAA_30M_REP1)
[f9/8ba4b8] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (WT_REP1)
[a4/62588e] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_UNINDUCED_REP2)
[8d/cb6ddb] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_UNINDUCED_REP1)
[bd/930813] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_UNINDUCED_REP1)
[dd/89f5da] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_UNINDUCED_REP2)
[e7/9c3c02] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_IAA_30M_REP1)
[eb/0b9b17] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_UNINDUCED_REP1)
[b5/88ec1a] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_UNINDUCED_REP2)
[ca/d7c7d2] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_IAA_30M_REP1)
[c2/3181ac] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_IAA_30M_REP1)
[a8/87508b] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:CUSTOM_TX2GENE (null)
[11/334e33] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_UNINDUCED_REP2)
[92/7ad4a9] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_IAA_30M_REP1)
[34/ad6173] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_UNINDUCED_REP1)
[40/3e6f77] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_IAA_30M_REP1)
[66/460eec] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_UNINDUCED_REP1)
[b4/4f66c3] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (WT_REP1)
[7b/ebc669] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_IAA_30M_REP1)
[22/02eb8b] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (WT_REP2)
[b2/30bf7b] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_UNINDUCED_REP2)
[ae/0020e1] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_UNINDUCED_REP1)
[41/610e47] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (WT_REP2)
[ea/851654] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_UNINDUCED_REP2)
[cf/a2a76d] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (WT_REP2)
[70/a6931c] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (WT_REP1)
[28/08c8a8] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (WT_REP1)
[44/ea51a6] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:TXIMETA_TXIMPORT
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[20/d38e40] Submitted process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_PSEUDO
[dd/8872ca] Submitted process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON
[94/c8ac1d] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SE_GENE (all_samples)
[8d/848df1] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SE_GENE_LENGTH_SCALED (all_samples)
[f0/1bc67e] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_TRANSCRIPT (all_samples)
[93/524a37] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SE_TRANSCRIPT (all_samples)
[82/61cd96] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_LENGTH_SCALED (all_samples)
[a2/987e76] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_SCALED (all_samples)
[71/020eeb] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SE_GENE_SCALED (all_samples)
[c1/8413d0] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE (all_samples)
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
WARN: Access to undefined parameter `enable_multiqc_ai` -- Initialise it to a default value eg. `params.enable_multiqc_ai = some_value`
[05/752f5c] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC (1)
-[nf-core/rnaseq] Pipeline completed successfully with skipped sampl(es)-
-[nf-core/rnaseq] Please check MultiQC report: 2/5 samples skipped since they failed STAR 90% mapped threshold.-
Nextflow stderr:

Check failure on line 1 in Test pipeline with a minimum mapped read threshold of 90

See this annotation in the file changed.

@github-actions github-actions / JUnit Test Report

Test pipeline with a minimum mapped read threshold of 90.Params: --min_mapped_reads 90 - stub

Assertion failed: 

1 of 2 assertions failed
Raw output
Nextflow stdout:

N E X T F L O W  ~  version 25.02.3-edge
Launching `/home/runner/work/rnaseq/rnaseq/tests/../main.nf` [nauseous_knuth] DSL2 - revision: 85c9b75b8b

------------------------------------------------------
                                        ,--./,-.
        ___     __   __   __   ___     /,-._.--~'
  |\ | |__  __ /  ` /  \ |__) |__         }  {
  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                        `._,._,'
  nf-core/rnaseq 3.19.0dev
------------------------------------------------------
Input/output options
  input                                              : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/samplesheet/v3.10/samplesheet_test.csv
  outdir                                             : /home/runner/work/rnaseq/rnaseq/~/tests/80f706261adbdfe9ea16a6a9f6ee16f1/output

Reference genome options
  fasta                                              : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genome.fasta
  gtf                                                : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes_with_empty_tid.gtf.gz
  gff                                                : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes.gff.gz
  transcript_fasta                                   : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/transcriptome.fasta
  additional_fasta                                   : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/gfp.fa.gz
  hisat2_index                                       : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/hisat2.tar.gz
  rsem_index                                         : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/rsem.tar.gz
  salmon_index                                       : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/salmon.tar.gz
  hisat2_build_memory                                : 3.GB

Read filtering options
  bbsplit_fasta_list                                 : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/bbsplit_fasta_list.txt

UMI options
  umitools_bc_pattern                                : NNNN

Alignment options
  pseudo_aligner                                     : salmon
  min_mapped_reads                                   : 90

Process skipping options
  skip_bbsplit                                       : false

Institutional config options
  config_profile_name                                : Test profile
  config_profile_description                         : Minimal test dataset to check pipeline function

Generic options
  pipelines_testdata_base_path                       : s3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/
  trace_report_suffix                                : 2025-04-11_20-03-46

Core Nextflow options
  runName                                            : nauseous_knuth
  containerEngine                                    : docker
  container.BBMAP_BBSPLIT                            : community.wave.seqera.io/library/bbmap:39.10--168578230ff6490a
  container.BEDTOOLS_GENOMECOV                       : community.wave.seqera.io/library/bedtools:2.31.1--7c4ce4cb07c09ee4
  container.BRACKEN_BRACKEN                          : community.wave.seqera.io/library/bracken:2.9--3d5af74ed6966fc1
  container.CAT_FASTQ                                : community.wave.seqera.io/library/coreutils:9.5--ae99c88a9b28c264
  container.CUSTOM_CATADDITIONALFASTA                : community.wave.seqera.io/library/python:3.12.2--8aba3d9ac529ff19
  container.CUSTOM_GETCHROMSIZES                     : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.CUSTOM_TX2GENE                           : community.wave.seqera.io/library/python:3.10.4--a643e28963f01eef
  container.DESEQ2_QC                                : community.wave.seqera.io/library/r-base_r-optparse_r-ggplot2_r-rcolorbrewer_pruned:d06fadc02f8422a2
  container.DUPRADAR                                 : community.wave.seqera.io/library/bioconductor-dupradar:1.32.0--dcb3a3be56be6612
  container.FASTP                                    : community.wave.seqera.io/library/fastp:0.23.4--f8cefc1e5f7a782e
  container.FASTQC                                   : community.wave.seqera.io/library/fastqc:0.12.1--af7a5314d5015c29
  container.FQ_LINT                                  : community.wave.seqera.io/library/fq:0.12.0--45ea0ea1c4d20daa
  container.FQ_SUBSAMPLE                             : community.wave.seqera.io/library/fq:0.12.0--45ea0ea1c4d20daa
  container.GFFREAD                                  : community.wave.seqera.io/library/gffread:0.12.7--33b95f1cfcc0e572
  container.GTF2BED                                  : community.wave.seqera.io/library/perl:5.26.2--e6570c5f08d3e12c
  container.GTF_FILTER                               : community.wave.seqera.io/library/python:3.9.5--0ee01a3faebf676a
  container.GUNZIP                                   : community.wave.seqera.io/library/grep_sed_tar:4f61255d5eff93dc
  container.HISAT2_ALIGN                             : community.wave.seqera.io/library/hisat2_samtools:9ef5e68250d2aa60
  container.HISAT2_BUILD                             : community.wave.seqera.io/library/hisat2:2.2.1--df34d2bb25ac6de5
  container.HISAT2_EXTRACTSPLICESITES                : community.wave.seqera.io/library/hisat2:2.2.1--df34d2bb25ac6de5
  container.KALLISTO_INDEX                           : community.wave.seqera.io/library/kallisto:0.51.1--b63691b6841c7a52
  container.KALLISTO_QUANT                           : community.wave.seqera.io/library/kallisto:0.51.1--b63691b6841c7a52
  container.KRAKEN2_KRAKEN2                          : community.wave.seqera.io/library/kraken2_coreutils_pigz:0892e153c7954635
  container.MULTIQC                                  : community.wave.seqera.io/library/multiqc:1.27--b0d1ffb40dfd9e97
  container.MULTIQC_CUSTOM_BIOTYPE                   : community.wave.seqera.io/library/python:3.9.5--0ee01a3faebf676a
  container.PICARD_MARKDUPLICATES                    : community.wave.seqera.io/library/picard:3.1.1--bae6cd265e6bf3d3
  container.PREPROCESS_TRANSCRIPTS_FASTA_GENCODE     : community.wave.seqera.io/library/sed:4.7--c9d1b71422302845
  container.PRESEQ_LCEXTRAP                          : community.wave.seqera.io/library/preseq:3.2.0--2789d8b704b33613
  container.QUALIMAP_RNASEQ                          : community.wave.seqera.io/library/qualimap:2.3--c1797c2253925b3a
  container.RSEM_CALCULATEEXPRESSION                 : community.wave.seqera.io/library/rsem_star:5acb4e8c03239c32
  container.RSEM_MERGE_COUNTS                        : community.wave.seqera.io/library/sed:4.7--c9d1b71422302845
  container.RSEM_PREPAREREFERENCE                    : community.wave.seqera.io/library/rsem_star:5acb4e8c03239c32
  container.RSEQC_BAMSTAT                            : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_INFEREXPERIMENT                    : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_INNERDISTANCE                      : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_JUNCTIONANNOTATION                 : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_JUNCTIONSATURATION                 : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_READDISTRIBUTION                   : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_READDUPLICATION                    : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_TIN                                : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.SALMON_INDEX                             : community.wave.seqera.io/library/salmon:1.10.3--fcd0755dd8abb423
  container.SALMON_QUANT                             : community.wave.seqera.io/library/salmon:1.10.3--fcd0755dd8abb423
  container.SAMTOOLS_FLAGSTAT                        : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_IDXSTATS                        : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_INDEX                           : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_SORT                            : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_STATS                           : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SORTMERNA                                : community.wave.seqera.io/library/sortmerna:4.3.7--6502243397c065ba
  container.STAR_ALIGN                               : community.wave.seqera.io/library/htslib_samtools_star_gawk:311d422a50e6d829
  container.STAR_ALIGN_IGENOMES                      : community.wave.seqera.io/library/star_samtools_gawk:bef5708b1c54889a
  container.STAR_GENOMEGENERATE                      : community.wave.seqera.io/library/htslib_samtools_star_gawk:311d422a50e6d829
  container.STAR_GENOMEGENERATE_IGENOMES             : community.wave.seqera.io/library/star_samtools_gawk:bef5708b1c54889a
  container.STRINGTIE_STRINGTIE                      : community.wave.seqera.io/library/stringtie:2.2.3--3c30041821e5a79a
  container.SUBREAD_FEATURECOUNTS                    : community.wave.seqera.io/library/subread:2.0.6--1968a7457b0ed34e
  container.SUMMARIZEDEXPERIMENT_SUMMARIZEDEXPERIMENT: community.wave.seqera.io/library/bioconductor-summarizedexperiment:1.32.0--13051b66560c2065
  container.TRIMGALORE                               : community.wave.seqera.io/library/cutadapt_trim-galore_pigz:a98edd405b34582d
  container.TXIMETA_TXIMPORT                         : community.wave.seqera.io/library/bioconductor-tximeta:1.20.1--cd025af46d4ef23a
  container.UCSC_BEDCLIP                             : community.wave.seqera.io/library/ucsc-bedclip:377--41de764ea1c26af4
  container.UCSC_BEDGRAPHTOBIGWIG                    : community.wave.seqera.io/library/ucsc-bedgraphtobigwig:469--f66c00635e28f216
  container.UMICOLLAPSE                              : community.wave.seqera.io/library/umicollapse:1.1.0--74864b350c72f3a0
  container.UMITOOLS_DEDUP                           : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UMITOOLS_EXTRACT                         : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UMITOOLS_PREPAREFORRSEM                  : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UNTAR                                    : community.wave.seqera.io/library/grep_sed_tar:4f61255d5eff93dc
  launchDir                                          : /home/runner/work/rnaseq/rnaseq/~/tests/80f706261adbdfe9ea16a6a9f6ee16f1
  workDir                                            : /home/runner/work/rnaseq/rnaseq/~/tests/80f706261adbdfe9ea16a6a9f6ee16f1/work
  projectDir                                         : /home/runner/work/rnaseq/rnaseq
  userName                                           : runner
  profile                                            : test,docker
  configFiles                                        : /home/runner/work/rnaseq/rnaseq/nextflow.config, /home/runner/work/rnaseq/rnaseq/nextflow.config, /home/runner/work/rnaseq/rnaseq/tests/nextflow.config

!! Only displaying parameters that differ from the pipeline defaults !!
------------------------------------------------------
* The pipeline
    https://doi.org/10.5281/zenodo.1400710

* The nf-core framework
    https://doi.org/10.1038/s41587-020-0439-x

* Software dependencies
    https://github.com/nf-core/rnaseq/blob/master/CITATIONS.md

WARN: The following invalid input values have been detected:

* --modules_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/modules/


WARN: nf-core pipelines do not accept positional arguments. The positional argument `true` has been detected.
HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.

WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  Both '--gtf' and '--gff' parameters have been provided.
  Using GTF file as priority.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  '--transcript_fasta' parameter has been provided.
  Make sure transcript names in this file match those in the GFF/GTF file.

  Please see:
  https://github.com/nf-core/rnaseq/issues/753
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
WARN: The operator `first` is useless when applied to a value channel which returns a single value by definition
WARN: The `when` process section is deprecated -- use conditional logic in the calling workflow instead
[39/6c5a92] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz)
[d9/2484dd] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:UNTAR_SALMON_INDEX (salmon.tar.gz)
[7b/53d1e1] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz)
[d7/312b16] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_UNINDUCED_REP1)
[8b/f82f47] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_IAA_30M_REP1)
[95/c3c574] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ (RAP1_UNINDUCED_REP2)
[f4/671a50] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (WT_REP2)
[1c/035814] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ (WT_REP1)
[49/7db606] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_UNINDUCED_REP1)
[da/bcfd21] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_UNINDUCED_REP1)
[37/4aab1b] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER (genome.fasta)
[00/a4db15] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_UNINDUCED_REP2)
[6a/07e1e7] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_IAA_30M_REP1)
[38/b40494] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_IAA_30M_REP1)
[ee/a85552] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (WT_REP1)
[33/0c2ff3] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP2)
[23/34c802] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP2)
[21/7e43b4] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_UNINDUCED_REP2)
[87/89858e] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_UNINDUCED_REP2)
[9d/99398c] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA (null)
WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  Biotype attribute 'gene_biotype' not found in the last column of the GTF file!

  Biotype QC will be skipped to circumvent the issue below:
  https://github.com/nf-core/rnaseq/issues/460

  Amend '--featurecounts_group_type' to change this behaviour.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
[73/8fe4ff] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP1)
[42/c9cafd] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP1)
[f1/2c810a] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED (genome_transcriptome.gtf)
[40/4a8e67] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome_transcriptome.fasta)
[e0/99ca3c] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:STAR_GENOMEGENERATE (genome_transcriptome.fasta)
[c7/447e5f] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT (null)
WARN: Access to undefined parameter `enable_multiqc_ai` -- Initialise it to a default value eg. `params.enable_multiqc_ai = some_value`
[c1/f7a191] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC (1)
-[nf-core/rnaseq] Pipeline completed successfully with skipped sampl(es)-
-[nf-core/rnaseq] Please check MultiQC report: 5/5 samples skipped since they failed 10000 trimmed read threshold.-
Nextflow stderr: