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ARM Megatests

ARM Megatests #336

GitHub Actions / JUnit Test Report failed Apr 11, 2025 in 0s

2 tests run, 0 passed, 0 skipped, 2 failed.

Annotations

Check failure on line 1 in Test pipeline with a minimum mapped read threshold of 90

See this annotation in the file changed.

@github-actions github-actions / JUnit Test Report

Test pipeline with a minimum mapped read threshold of 90.Params: --min_mapped_reads 90

Assertion failed: 

1 of 2 assertions failed
Raw output
Nextflow stdout:

N E X T F L O W  ~  version 24.04.2
Launching `/home/runner/work/rnaseq/rnaseq/tests/../main.nf` [sharp_ardinghelli] DSL2 - revision: 85c9b75b8b
Downloading plugin [email protected]
WARN: Invalid config manifest attribute `contributors`

------------------------------------------------------
                                        ,--./,-.
        ___     __   __   __   ___     /,-._.--~'
  |\ | |__  __ /  ` /  \ |__) |__         }  {
  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                        `._,._,'
  nf-core/rnaseq 3.19.0dev
------------------------------------------------------
Input/output options
  input                                              : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/samplesheet/v3.10/samplesheet_test.csv
  outdir                                             : /home/runner/work/rnaseq/rnaseq/~/tests/716928385ff6315bd23e989a7cf7e4a1/output

Reference genome options
  fasta                                              : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genome.fasta
  gtf                                                : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes_with_empty_tid.gtf.gz
  gff                                                : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes.gff.gz
  transcript_fasta                                   : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/transcriptome.fasta
  additional_fasta                                   : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/gfp.fa.gz
  hisat2_index                                       : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/hisat2.tar.gz
  rsem_index                                         : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/rsem.tar.gz
  salmon_index                                       : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/salmon.tar.gz
  hisat2_build_memory                                : 3.GB

Read filtering options
  bbsplit_fasta_list                                 : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/bbsplit_fasta_list.txt

UMI options
  umitools_bc_pattern                                : NNNN

Alignment options
  pseudo_aligner                                     : salmon
  min_mapped_reads                                   : 90

Process skipping options
  skip_bbsplit                                       : false

Institutional config options
  config_profile_name                                : Test profile
  config_profile_description                         : Minimal test dataset to check pipeline function

Generic options
  pipelines_testdata_base_path                       : s3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/
  trace_report_suffix                                : 2025-04-11_19-51-38

Core Nextflow options
  runName                                            : sharp_ardinghelli
  containerEngine                                    : docker
  container.BBMAP_BBSPLIT                            : community.wave.seqera.io/library/bbmap:39.10--168578230ff6490a
  container.BEDTOOLS_GENOMECOV                       : community.wave.seqera.io/library/bedtools:2.31.1--7c4ce4cb07c09ee4
  container.BRACKEN_BRACKEN                          : community.wave.seqera.io/library/bracken:2.9--3d5af74ed6966fc1
  container.CAT_FASTQ                                : community.wave.seqera.io/library/coreutils:9.5--ae99c88a9b28c264
  container.CUSTOM_CATADDITIONALFASTA                : community.wave.seqera.io/library/python:3.12.2--8aba3d9ac529ff19
  container.CUSTOM_GETCHROMSIZES                     : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.CUSTOM_TX2GENE                           : community.wave.seqera.io/library/python:3.10.4--a643e28963f01eef
  container.DESEQ2_QC                                : community.wave.seqera.io/library/r-base_r-optparse_r-ggplot2_r-rcolorbrewer_pruned:d06fadc02f8422a2
  container.DUPRADAR                                 : community.wave.seqera.io/library/bioconductor-dupradar:1.32.0--dcb3a3be56be6612
  container.FASTP                                    : community.wave.seqera.io/library/fastp:0.23.4--f8cefc1e5f7a782e
  container.FASTQC                                   : community.wave.seqera.io/library/fastqc:0.12.1--af7a5314d5015c29
  container.FQ_LINT                                  : community.wave.seqera.io/library/fq:0.12.0--45ea0ea1c4d20daa
  container.FQ_SUBSAMPLE                             : community.wave.seqera.io/library/fq:0.12.0--45ea0ea1c4d20daa
  container.GFFREAD                                  : community.wave.seqera.io/library/gffread:0.12.7--33b95f1cfcc0e572
  container.GTF2BED                                  : community.wave.seqera.io/library/perl:5.26.2--e6570c5f08d3e12c
  container.GTF_FILTER                               : community.wave.seqera.io/library/python:3.9.5--0ee01a3faebf676a
  container.GUNZIP                                   : community.wave.seqera.io/library/grep_sed_tar:4f61255d5eff93dc
  container.HISAT2_ALIGN                             : community.wave.seqera.io/library/hisat2_samtools:9ef5e68250d2aa60
  container.HISAT2_BUILD                             : community.wave.seqera.io/library/hisat2:2.2.1--df34d2bb25ac6de5
  container.HISAT2_EXTRACTSPLICESITES                : community.wave.seqera.io/library/hisat2:2.2.1--df34d2bb25ac6de5
  container.KALLISTO_INDEX                           : community.wave.seqera.io/library/kallisto:0.51.1--b63691b6841c7a52
  container.KALLISTO_QUANT                           : community.wave.seqera.io/library/kallisto:0.51.1--b63691b6841c7a52
  container.KRAKEN2_KRAKEN2                          : community.wave.seqera.io/library/kraken2_coreutils_pigz:0892e153c7954635
  container.MULTIQC                                  : community.wave.seqera.io/library/multiqc:1.27--b0d1ffb40dfd9e97
  container.MULTIQC_CUSTOM_BIOTYPE                   : community.wave.seqera.io/library/python:3.9.5--0ee01a3faebf676a
  container.PICARD_MARKDUPLICATES                    : community.wave.seqera.io/library/picard:3.1.1--bae6cd265e6bf3d3
  container.PREPROCESS_TRANSCRIPTS_FASTA_GENCODE     : community.wave.seqera.io/library/sed:4.7--c9d1b71422302845
  container.PRESEQ_LCEXTRAP                          : community.wave.seqera.io/library/preseq:3.2.0--2789d8b704b33613
  container.QUALIMAP_RNASEQ                          : community.wave.seqera.io/library/qualimap:2.3--c1797c2253925b3a
  container.RSEM_CALCULATEEXPRESSION                 : community.wave.seqera.io/library/rsem_star:5acb4e8c03239c32
  container.RSEM_MERGE_COUNTS                        : community.wave.seqera.io/library/sed:4.7--c9d1b71422302845
  container.RSEM_PREPAREREFERENCE                    : community.wave.seqera.io/library/rsem_star:5acb4e8c03239c32
  container.RSEQC_BAMSTAT                            : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_INFEREXPERIMENT                    : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_INNERDISTANCE                      : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_JUNCTIONANNOTATION                 : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_JUNCTIONSATURATION                 : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_READDISTRIBUTION                   : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_READDUPLICATION                    : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_TIN                                : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.SALMON_INDEX                             : community.wave.seqera.io/library/salmon:1.10.3--fcd0755dd8abb423
  container.SALMON_QUANT                             : community.wave.seqera.io/library/salmon:1.10.3--fcd0755dd8abb423
  container.SAMTOOLS_FLAGSTAT                        : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_IDXSTATS                        : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_INDEX                           : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_SORT                            : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_STATS                           : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SORTMERNA                                : community.wave.seqera.io/library/sortmerna:4.3.7--6502243397c065ba
  container.STAR_ALIGN                               : community.wave.seqera.io/library/htslib_samtools_star_gawk:311d422a50e6d829
  container.STAR_ALIGN_IGENOMES                      : community.wave.seqera.io/library/star_samtools_gawk:bef5708b1c54889a
  container.STAR_GENOMEGENERATE                      : community.wave.seqera.io/library/htslib_samtools_star_gawk:311d422a50e6d829
  container.STAR_GENOMEGENERATE_IGENOMES             : community.wave.seqera.io/library/star_samtools_gawk:bef5708b1c54889a
  container.STRINGTIE_STRINGTIE                      : community.wave.seqera.io/library/stringtie:2.2.3--3c30041821e5a79a
  container.SUBREAD_FEATURECOUNTS                    : community.wave.seqera.io/library/subread:2.0.6--1968a7457b0ed34e
  container.SUMMARIZEDEXPERIMENT_SUMMARIZEDEXPERIMENT: community.wave.seqera.io/library/bioconductor-summarizedexperiment:1.32.0--13051b66560c2065
  container.TRIMGALORE                               : community.wave.seqera.io/library/cutadapt_trim-galore_pigz:a98edd405b34582d
  container.TXIMETA_TXIMPORT                         : community.wave.seqera.io/library/bioconductor-tximeta:1.20.1--cd025af46d4ef23a
  container.UCSC_BEDCLIP                             : community.wave.seqera.io/library/ucsc-bedclip:377--41de764ea1c26af4
  container.UCSC_BEDGRAPHTOBIGWIG                    : community.wave.seqera.io/library/ucsc-bedgraphtobigwig:469--f66c00635e28f216
  container.UMICOLLAPSE                              : community.wave.seqera.io/library/umicollapse:1.1.0--74864b350c72f3a0
  container.UMITOOLS_DEDUP                           : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UMITOOLS_EXTRACT                         : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UMITOOLS_PREPAREFORRSEM                  : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UNTAR                                    : community.wave.seqera.io/library/grep_sed_tar:4f61255d5eff93dc
  launchDir                                          : /home/runner/work/rnaseq/rnaseq/~/tests/716928385ff6315bd23e989a7cf7e4a1
  workDir                                            : /home/runner/work/rnaseq/rnaseq/~/tests/716928385ff6315bd23e989a7cf7e4a1/work
  projectDir                                         : /home/runner/work/rnaseq/rnaseq
  userName                                           : runner
  profile                                            : test,docker
  configFiles                                        : /home/runner/work/rnaseq/rnaseq/nextflow.config, /home/runner/work/rnaseq/rnaseq/nextflow.config, /home/runner/work/rnaseq/rnaseq/tests/nextflow.config

!! Only displaying parameters that differ from the pipeline defaults !!
------------------------------------------------------
* The pipeline
    https://doi.org/10.5281/zenodo.1400710

* The nf-core framework
    https://doi.org/10.1038/s41587-020-0439-x

* Software dependencies
    https://github.com/nf-core/rnaseq/blob/master/CITATIONS.md

WARN: The following invalid input values have been detected:

* --modules_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/modules/


WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  Both '--gtf' and '--gff' parameters have been provided.
  Using GTF file as priority.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  '--transcript_fasta' parameter has been provided.
  Make sure transcript names in this file match those in the GFF/GTF file.

  Please see:
  https://github.com/nf-core/rnaseq/issues/753
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
WARN: The operator `first` is useless when applied to a value channel which returns a single value by definition
[1a/f75c1f] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz)
[24/283ee1] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:UNTAR_SALMON_INDEX (salmon.tar.gz)
[31/4d75e1] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_UNINDUCED_REP1)
[fc/285f55] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz)
[be/1358c8] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ (RAP1_UNINDUCED_REP2)
[84/b2dd8d] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ (WT_REP1)
[56/0c1caf] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (WT_REP2)
[ba/662da8] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_IAA_30M_REP1)
[a4/cfba9b] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER (genome.fasta)
[f3/2af135] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_UNINDUCED_REP1)
[07/e10717] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_UNINDUCED_REP1)
[d0/7f782b] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_UNINDUCED_REP2)
[9d/3d0629] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP2)
[6b/b9618e] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP2)
[db/9a79bd] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (WT_REP1)
[bc/5b3e68] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_IAA_30M_REP1)
[98/47496a] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_IAA_30M_REP1)
[28/1cc07a] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA (null)
[55/9b3b17] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_UNINDUCED_REP2)
[9a/92c79c] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_UNINDUCED_REP2)
[25/97e99e] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (RAP1_UNINDUCED_REP1)
[46/3df3ab] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP1)
[79/e9cb77] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP1)
[26/dccc61] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (WT_REP2)
[a6/7b92ed] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (RAP1_IAA_30M_REP1)
[0b/d79f5c] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED (genome_gfp.gtf)
[ea/c8a2ff] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome_gfp.fasta)
[d8/5c2d93] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:STAR_GENOMEGENERATE (genome_gfp.fasta)
[bc/869600] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT (null)
[61/9ff70a] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (RAP1_UNINDUCED_REP2)
[65/c5b5ef] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_TRIMMING (WT_REP1)
[65/1a8539] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (WT_REP2)
[2e/4ddf5f] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (RAP1_UNINDUCED_REP1)
[68/29d7fc] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (RAP1_IAA_30M_REP1)
[61/931ff3] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (RAP1_UNINDUCED_REP2)
[88/12abd6] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:BBMAP_BBSPLIT (WT_REP1)
[63/4d785d] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (RAP1_UNINDUCED_REP1)
[fe/704e8d] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (WT_REP2)
[c4/419ed3] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (RAP1_IAA_30M_REP1)
[46/43c0bd] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (RAP1_UNINDUCED_REP2)
[76/f75cec] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (RAP1_UNINDUCED_REP1)
[da/f9ed83] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_UNINDUCED_REP1)
[85/862a84] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP2)
[f2/147222] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (WT_REP2)
[6f/9708d7] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (RAP1_IAA_30M_REP1)
[14/36ae20] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_IAA_30M_REP1)
[89/7f55e3] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_UNINDUCED_REP2)
[bd/9f1f41] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (RAP1_UNINDUCED_REP2)
[1e/f60207] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (RAP1_UNINDUCED_REP1)
[ad/6c526b] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (RAP1_UNINDUCED_REP1)
[82/41b4ab] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT_AFTER_BBSPLIT (WT_REP1)
[22/1bfaa9] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (WT_REP2)
[9f/b8c29e] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (WT_REP2)
[da/0803e9] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (RAP1_IAA_30M_REP1)
[14/4d60d3] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (RAP1_IAA_30M_REP1)
[d4/12115b] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (RAP1_UNINDUCED_REP1)
[3e/76acda] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES (RAP1_UNINDUCED_REP1)
[94/40fa38] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:FQ_SUBSAMPLE (WT_REP1)
[04/add14a] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (WT_REP2)
[dc/ab20f7] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (RAP1_IAA_30M_REP1)
[ad/650e4b] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES (RAP1_IAA_30M_REP1)
[ed/76021c] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (RAP1_UNINDUCED_REP2)
[72/4b7372] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (RAP1_UNINDUCED_REP2)
[4e/3736c3] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:SALMON_QUANT (WT_REP1)
[f3/38e6a2] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_FW (RAP1_UNINDUCED_REP1)
[21/60fef3] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_REV (RAP1_UNINDUCED_REP1)
[a2/ec3a34] Submitted process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE (RAP1_UNINDUCED_REP1)
[d4/ad5707] Submitted process > NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ (RAP1_UNINDUCED_REP1)
[dc/e227a2] Submitted process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS (RAP1_UNINDUCED_REP1)
[c9/43bf35] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX (RAP1_UNINDUCED_REP1)
[f7/e4f6e6] Submitted process > NFCORE_RNASEQ:RNASEQ:DUPRADAR (RAP1_UNINDUCED_REP1)
[0d/fb4824] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES (RAP1_UNINDUCED_REP2)
[49/5778ed] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (RAP1_UNINDUCED_REP2)
[e1/968590] Submitted process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS (RAP1_IAA_30M_REP1)
[5d/b45328] Submitted process > NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ (RAP1_IAA_30M_REP1)
[c8/22d1f3] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX (RAP1_IAA_30M_REP1)
[d2/271884] Submitted process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE (RAP1_IAA_30M_REP1)
[09/3d46d7] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_FW (RAP1_IAA_30M_REP1)
[8b/6a8674] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_REV (RAP1_IAA_30M_REP1)
[38/b06ce2] Submitted process > NFCORE_RNASEQ:RNASEQ:DUPRADAR (RAP1_IAA_30M_REP1)
[1e/30f092] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SALMON_QUANT (WT_REP1)
[b4/418274] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)
[46/297706] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDCLIP (RAP1_UNINDUCED_REP1)
[ef/afb0c5] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDCLIP (RAP1_UNINDUCED_REP1)
[ab/478d2e] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE (RAP1_UNINDUCED_REP1)
[ed/db5824] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_BAMSTAT (RAP1_UNINDUCED_REP1)
[77/7143d1] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDISTRIBUTION (RAP1_UNINDUCED_REP1)
[90/2e8ab5] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONSATURATION (RAP1_UNINDUCED_REP1)
[69/1fee40] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDUPLICATION (RAP1_UNINDUCED_REP1)
[0e/d574eb] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INFEREXPERIMENT (RAP1_UNINDUCED_REP1)
[04/7f7238] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INNERDISTANCE (RAP1_UNINDUCED_REP1)
[92/058343] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONANNOTATION (RAP1_UNINDUCED_REP1)
[b6/d7f6a8] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX (RAP1_UNINDUCED_REP2)
[a8/07fa9d] Submitted process > NFCORE_RNASEQ:RNASEQ:QUALIMAP_RNASEQ (RAP1_UNINDUCED_REP2)
[08/ccecff] Submitted process > NFCORE_RNASEQ:RNASEQ:STRINGTIE_STRINGTIE (RAP1_UNINDUCED_REP2)
[0c/114068] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_FW (RAP1_UNINDUCED_REP2)
[2e/9cfbb8] Submitted process > NFCORE_RNASEQ:RNASEQ:SUBREAD_FEATURECOUNTS (RAP1_UNINDUCED_REP2)
[4b/ed5c6a] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDTOOLS_GENOMECOV_REV (RAP1_UNINDUCED_REP2)
[75/e37325] Submitted process > NFCORE_RNASEQ:RNASEQ:DUPRADAR (RAP1_UNINDUCED_REP2)
[35/21335e] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE (RAP1_IAA_30M_REP1)
[06/8b99a5] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDISTRIBUTION (RAP1_IAA_30M_REP1)
[85/151bca] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INFEREXPERIMENT (RAP1_IAA_30M_REP1)
[12/997c21] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONANNOTATION (RAP1_IAA_30M_REP1)
[93/b094df] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_BAMSTAT (RAP1_IAA_30M_REP1)
[ed/e30cb0] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONSATURATION (RAP1_IAA_30M_REP1)
[7d/0a8003] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDUPLICATION (RAP1_IAA_30M_REP1)
[c6/eb7e27] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INNERDISTANCE (RAP1_IAA_30M_REP1)
[66/d376a7] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDCLIP (RAP1_IAA_30M_REP1)
[14/866a8d] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDCLIP (RAP1_IAA_30M_REP1)
[37/4b0502] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:CUSTOM_TX2GENE (null)
[12/f316ba] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG (RAP1_UNINDUCED_REP1)
[24/f1f416] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG (RAP1_UNINDUCED_REP1)
[9b/867af2] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDUPLICATION (RAP1_UNINDUCED_REP2)
[42/44fa15] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_BAMSTAT (RAP1_UNINDUCED_REP2)
[e6/d78171] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INFEREXPERIMENT (RAP1_UNINDUCED_REP2)
[e5/089746] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_INNERDISTANCE (RAP1_UNINDUCED_REP2)
[e3/b5f9c6] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONANNOTATION (RAP1_UNINDUCED_REP2)
[c3/6df992] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONSATURATION (RAP1_UNINDUCED_REP2)
[54/6c0b53] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_READDISTRIBUTION (RAP1_UNINDUCED_REP2)
[64/0cc9b3] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDCLIP (RAP1_UNINDUCED_REP2)
[8e/c16c58] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC_CUSTOM_BIOTYPE (RAP1_UNINDUCED_REP2)
[4c/7eed0c] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDCLIP (RAP1_UNINDUCED_REP2)
[7b/ed1403] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG (RAP1_IAA_30M_REP1)
[91/f70304] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG (RAP1_IAA_30M_REP1)
[d8/876d19] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:TXIMETA_TXIMPORT
[17/e0b704] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_FORWARD:UCSC_BEDGRAPHTOBIGWIG (RAP1_UNINDUCED_REP2)
[c7/d970ab] Submitted process > NFCORE_RNASEQ:RNASEQ:BEDGRAPH_BEDCLIP_BEDGRAPHTOBIGWIG_REVERSE:UCSC_BEDGRAPHTOBIGWIG (RAP1_UNINDUCED_REP2)
[86/13d163] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT (WT_REP1)
[eb/9a787b] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SALMON_QUANT (WT_REP1)
[71/0216cd] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX (WT_REP1)
[d0/4ed4b4] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_UNINDUCED_REP1)
[b6/fe0a22] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_IAA_30M_REP1)
[82/2f65fa] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_IAA_30M_REP1)
[c8/47e2e4] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_UNINDUCED_REP2)
[bc/04cd9e] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_UNINDUCED_REP2)
[23/72d24e] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_IAA_30M_REP1)
[5e/f91231] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_UNINDUCED_REP1)
[49/6314f0] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_UNINDUCED_REP2)
[4a/e3d63a] Submitted process > NFCORE_RNASEQ:RNASEQ:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_UNINDUCED_REP1)
[e3/c75f8b] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:CUSTOM_TX2GENE (null)
[2b/0e627a] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (WT_REP2)
[70/38874f] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_IAA_30M_REP1)
[4c/d2cccf] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_UNINDUCED_REP1)
[7e/bc9435] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (WT_REP2)
[e4/e088ab] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_UNINDUCED_REP1)
[70/00aaac] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (WT_REP2)
[42/30a827] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_UNINDUCED_REP1)
[a8/841acd] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (WT_REP1)
[6e/56bc73] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_IAA_30M_REP1)
[42/0a6be5] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_IDXSTATS (RAP1_UNINDUCED_REP2)
[80/8f8c70] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_UNINDUCED_REP2)
[ab/850491] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (RAP1_IAA_30M_REP1)
[db/6546b6] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_STATS (WT_REP1)
[ef/3a7e78] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (RAP1_UNINDUCED_REP2)
[4f/f8020a] Submitted process > NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_FLAGSTAT (WT_REP1)
[25/cbd141] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:TXIMETA_TXIMPORT
[74/67b6fc] Submitted process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_PSEUDO
[c9/50f1f0] Submitted process > NFCORE_RNASEQ:RNASEQ:DESEQ2_QC_STAR_SALMON
[30/fefab8] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SE_GENE_SCALED (all_samples)
[86/fcbddc] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SE_GENE (all_samples)
[75/06da11] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SE_TRANSCRIPT (all_samples)
[f5/7d3d22] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE (all_samples)
[5b/68ae17] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_LENGTH_SCALED (all_samples)
[9b/d81dab] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_PSEUDO_ALIGNMENT:SE_GENE_LENGTH_SCALED (all_samples)
WARN: Access to undefined parameter `enable_multiqc_ai` -- Initialise it to a default value eg. `params.enable_multiqc_ai = some_value`
[a4/4f7849] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_GENE_SCALED (all_samples)
[95/5c3f1f] Submitted process > NFCORE_RNASEQ:RNASEQ:QUANTIFY_STAR_SALMON:SE_TRANSCRIPT (all_samples)
[70/77c3e3] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC (1)
-[nf-core/rnaseq] Pipeline completed successfully with skipped sampl(es)-
-[nf-core/rnaseq] Please check MultiQC report: 2/5 samples skipped since they failed STAR 90% mapped threshold.-
Nextflow stderr:

Nextflow 24.10.5 is available - Please consider updating your version to it

Check failure on line 1 in Test pipeline with a minimum mapped read threshold of 90

See this annotation in the file changed.

@github-actions github-actions / JUnit Test Report

Test pipeline with a minimum mapped read threshold of 90.Params: --min_mapped_reads 90 - stub

Assertion failed: 

1 of 2 assertions failed
Raw output
Nextflow stdout:

N E X T F L O W  ~  version 24.04.2
Launching `/home/runner/work/rnaseq/rnaseq/tests/../main.nf` [condescending_escher] DSL2 - revision: 85c9b75b8b
WARN: Invalid config manifest attribute `contributors`

------------------------------------------------------
                                        ,--./,-.
        ___     __   __   __   ___     /,-._.--~'
  |\ | |__  __ /  ` /  \ |__) |__         }  {
  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                        `._,._,'
  nf-core/rnaseq 3.19.0dev
------------------------------------------------------
Input/output options
  input                                              : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/samplesheet/v3.10/samplesheet_test.csv
  outdir                                             : /home/runner/work/rnaseq/rnaseq/~/tests/80f706261adbdfe9ea16a6a9f6ee16f1/output

Reference genome options
  fasta                                              : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genome.fasta
  gtf                                                : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes_with_empty_tid.gtf.gz
  gff                                                : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/genes.gff.gz
  transcript_fasta                                   : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/transcriptome.fasta
  additional_fasta                                   : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/gfp.fa.gz
  hisat2_index                                       : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/hisat2.tar.gz
  rsem_index                                         : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/rsem.tar.gz
  salmon_index                                       : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/salmon.tar.gz
  hisat2_build_memory                                : 3.GB

Read filtering options
  bbsplit_fasta_list                                 : https://raw.githubusercontent.com/nf-core/test-datasets/626c8fab639062eade4b10747e919341cbf9b41a/reference/bbsplit_fasta_list.txt

UMI options
  umitools_bc_pattern                                : NNNN

Alignment options
  pseudo_aligner                                     : salmon
  min_mapped_reads                                   : 90

Process skipping options
  skip_bbsplit                                       : false

Institutional config options
  config_profile_name                                : Test profile
  config_profile_description                         : Minimal test dataset to check pipeline function

Generic options
  pipelines_testdata_base_path                       : s3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/
  trace_report_suffix                                : 2025-04-11_20-03-39

Core Nextflow options
  runName                                            : condescending_escher
  containerEngine                                    : docker
  container.BBMAP_BBSPLIT                            : community.wave.seqera.io/library/bbmap:39.10--168578230ff6490a
  container.BEDTOOLS_GENOMECOV                       : community.wave.seqera.io/library/bedtools:2.31.1--7c4ce4cb07c09ee4
  container.BRACKEN_BRACKEN                          : community.wave.seqera.io/library/bracken:2.9--3d5af74ed6966fc1
  container.CAT_FASTQ                                : community.wave.seqera.io/library/coreutils:9.5--ae99c88a9b28c264
  container.CUSTOM_CATADDITIONALFASTA                : community.wave.seqera.io/library/python:3.12.2--8aba3d9ac529ff19
  container.CUSTOM_GETCHROMSIZES                     : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.CUSTOM_TX2GENE                           : community.wave.seqera.io/library/python:3.10.4--a643e28963f01eef
  container.DESEQ2_QC                                : community.wave.seqera.io/library/r-base_r-optparse_r-ggplot2_r-rcolorbrewer_pruned:d06fadc02f8422a2
  container.DUPRADAR                                 : community.wave.seqera.io/library/bioconductor-dupradar:1.32.0--dcb3a3be56be6612
  container.FASTP                                    : community.wave.seqera.io/library/fastp:0.23.4--f8cefc1e5f7a782e
  container.FASTQC                                   : community.wave.seqera.io/library/fastqc:0.12.1--af7a5314d5015c29
  container.FQ_LINT                                  : community.wave.seqera.io/library/fq:0.12.0--45ea0ea1c4d20daa
  container.FQ_SUBSAMPLE                             : community.wave.seqera.io/library/fq:0.12.0--45ea0ea1c4d20daa
  container.GFFREAD                                  : community.wave.seqera.io/library/gffread:0.12.7--33b95f1cfcc0e572
  container.GTF2BED                                  : community.wave.seqera.io/library/perl:5.26.2--e6570c5f08d3e12c
  container.GTF_FILTER                               : community.wave.seqera.io/library/python:3.9.5--0ee01a3faebf676a
  container.GUNZIP                                   : community.wave.seqera.io/library/grep_sed_tar:4f61255d5eff93dc
  container.HISAT2_ALIGN                             : community.wave.seqera.io/library/hisat2_samtools:9ef5e68250d2aa60
  container.HISAT2_BUILD                             : community.wave.seqera.io/library/hisat2:2.2.1--df34d2bb25ac6de5
  container.HISAT2_EXTRACTSPLICESITES                : community.wave.seqera.io/library/hisat2:2.2.1--df34d2bb25ac6de5
  container.KALLISTO_INDEX                           : community.wave.seqera.io/library/kallisto:0.51.1--b63691b6841c7a52
  container.KALLISTO_QUANT                           : community.wave.seqera.io/library/kallisto:0.51.1--b63691b6841c7a52
  container.KRAKEN2_KRAKEN2                          : community.wave.seqera.io/library/kraken2_coreutils_pigz:0892e153c7954635
  container.MULTIQC                                  : community.wave.seqera.io/library/multiqc:1.27--b0d1ffb40dfd9e97
  container.MULTIQC_CUSTOM_BIOTYPE                   : community.wave.seqera.io/library/python:3.9.5--0ee01a3faebf676a
  container.PICARD_MARKDUPLICATES                    : community.wave.seqera.io/library/picard:3.1.1--bae6cd265e6bf3d3
  container.PREPROCESS_TRANSCRIPTS_FASTA_GENCODE     : community.wave.seqera.io/library/sed:4.7--c9d1b71422302845
  container.PRESEQ_LCEXTRAP                          : community.wave.seqera.io/library/preseq:3.2.0--2789d8b704b33613
  container.QUALIMAP_RNASEQ                          : community.wave.seqera.io/library/qualimap:2.3--c1797c2253925b3a
  container.RSEM_CALCULATEEXPRESSION                 : community.wave.seqera.io/library/rsem_star:5acb4e8c03239c32
  container.RSEM_MERGE_COUNTS                        : community.wave.seqera.io/library/sed:4.7--c9d1b71422302845
  container.RSEM_PREPAREREFERENCE                    : community.wave.seqera.io/library/rsem_star:5acb4e8c03239c32
  container.RSEQC_BAMSTAT                            : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_INFEREXPERIMENT                    : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_INNERDISTANCE                      : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_JUNCTIONANNOTATION                 : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_JUNCTIONSATURATION                 : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_READDISTRIBUTION                   : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_READDUPLICATION                    : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.RSEQC_TIN                                : community.wave.seqera.io/library/rseqc_r-base:2c6374f25c7b060e
  container.SALMON_INDEX                             : community.wave.seqera.io/library/salmon:1.10.3--fcd0755dd8abb423
  container.SALMON_QUANT                             : community.wave.seqera.io/library/salmon:1.10.3--fcd0755dd8abb423
  container.SAMTOOLS_FLAGSTAT                        : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_IDXSTATS                        : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_INDEX                           : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_SORT                            : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SAMTOOLS_STATS                           : community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf
  container.SORTMERNA                                : community.wave.seqera.io/library/sortmerna:4.3.7--6502243397c065ba
  container.STAR_ALIGN                               : community.wave.seqera.io/library/htslib_samtools_star_gawk:311d422a50e6d829
  container.STAR_ALIGN_IGENOMES                      : community.wave.seqera.io/library/star_samtools_gawk:bef5708b1c54889a
  container.STAR_GENOMEGENERATE                      : community.wave.seqera.io/library/htslib_samtools_star_gawk:311d422a50e6d829
  container.STAR_GENOMEGENERATE_IGENOMES             : community.wave.seqera.io/library/star_samtools_gawk:bef5708b1c54889a
  container.STRINGTIE_STRINGTIE                      : community.wave.seqera.io/library/stringtie:2.2.3--3c30041821e5a79a
  container.SUBREAD_FEATURECOUNTS                    : community.wave.seqera.io/library/subread:2.0.6--1968a7457b0ed34e
  container.SUMMARIZEDEXPERIMENT_SUMMARIZEDEXPERIMENT: community.wave.seqera.io/library/bioconductor-summarizedexperiment:1.32.0--13051b66560c2065
  container.TRIMGALORE                               : community.wave.seqera.io/library/cutadapt_trim-galore_pigz:a98edd405b34582d
  container.TXIMETA_TXIMPORT                         : community.wave.seqera.io/library/bioconductor-tximeta:1.20.1--cd025af46d4ef23a
  container.UCSC_BEDCLIP                             : community.wave.seqera.io/library/ucsc-bedclip:377--41de764ea1c26af4
  container.UCSC_BEDGRAPHTOBIGWIG                    : community.wave.seqera.io/library/ucsc-bedgraphtobigwig:469--f66c00635e28f216
  container.UMICOLLAPSE                              : community.wave.seqera.io/library/umicollapse:1.1.0--74864b350c72f3a0
  container.UMITOOLS_DEDUP                           : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UMITOOLS_EXTRACT                         : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UMITOOLS_PREPAREFORRSEM                  : community.wave.seqera.io/library/umi_tools:1.1.5--a4e9770134f31e60
  container.UNTAR                                    : community.wave.seqera.io/library/grep_sed_tar:4f61255d5eff93dc
  launchDir                                          : /home/runner/work/rnaseq/rnaseq/~/tests/80f706261adbdfe9ea16a6a9f6ee16f1
  workDir                                            : /home/runner/work/rnaseq/rnaseq/~/tests/80f706261adbdfe9ea16a6a9f6ee16f1/work
  projectDir                                         : /home/runner/work/rnaseq/rnaseq
  userName                                           : runner
  profile                                            : test,docker
  configFiles                                        : /home/runner/work/rnaseq/rnaseq/nextflow.config, /home/runner/work/rnaseq/rnaseq/nextflow.config, /home/runner/work/rnaseq/rnaseq/tests/nextflow.config

!! Only displaying parameters that differ from the pipeline defaults !!
------------------------------------------------------
* The pipeline
    https://doi.org/10.5281/zenodo.1400710

* The nf-core framework
    https://doi.org/10.1038/s41587-020-0439-x

* Software dependencies
    https://github.com/nf-core/rnaseq/blob/master/CITATIONS.md

WARN: The following invalid input values have been detected:

* --modules_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/modules/


WARN: nf-core pipelines do not accept positional arguments. The positional argument `true` has been detected.
HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.

WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  Both '--gtf' and '--gff' parameters have been provided.
  Using GTF file as priority.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  '--transcript_fasta' parameter has been provided.
  Make sure transcript names in this file match those in the GFF/GTF file.

  Please see:
  https://github.com/nf-core/rnaseq/issues/753
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
WARN: The operator `first` is useless when applied to a value channel which returns a single value by definition
[e9/c26a85] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_ADDITIONAL_FASTA (gfp.fa.gz)
[89/6dd039] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GUNZIP_GTF (genes_with_empty_tid.gtf.gz)
[4e/15d77a] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:UNTAR_SALMON_INDEX (salmon.tar.gz)
[94/ab3def] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_UNINDUCED_REP1)
[7f/3432d8] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (WT_REP2)
[aa/651c1b] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ (RAP1_UNINDUCED_REP2)
[13/184dbc] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_IAA_30M_REP1)
[39/efa428] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:CAT_FASTQ (WT_REP1)
[e9/c3bab0] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GTF_FILTER (genome.fasta)
[7f/22e217] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_UNINDUCED_REP1)
[ba/451c07] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_UNINDUCED_REP1)
[da/36a4f2] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP2)
[30/8f46d8] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP2)
[cf/3634af] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (RAP1_UNINDUCED_REP2)
[92/59a604] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_IAA_30M_REP1)
[4a/107194] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_IAA_30M_REP1)
[cd/297e22] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FQ_LINT (WT_REP1)
[38/6989a5] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_CATADDITIONALFASTA (null)
[7b/b34443] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (RAP1_UNINDUCED_REP2)
[a4/9862e0] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (RAP1_UNINDUCED_REP2)
WARN: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
  Biotype attribute 'gene_biotype' not found in the last column of the GTF file!

  Biotype QC will be skipped to circumvent the issue below:
  https://github.com/nf-core/rnaseq/issues/460

  Amend '--featurecounts_group_type' to change this behaviour.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
[b1/c60901] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:FASTQC (WT_REP1)
[e5/047dbc] Submitted process > NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT_REP1)
[6a/3fac7b] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (genome_transcriptome.fasta)
[42/98db30] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:GTF2BED (genome_transcriptome.gtf)
[ee/428c89] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:STAR_GENOMEGENERATE (genome_transcriptome.fasta)
[e9/9d383d] Submitted process > NFCORE_RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT (null)
WARN: Access to undefined parameter `enable_multiqc_ai` -- Initialise it to a default value eg. `params.enable_multiqc_ai = some_value`
[47/3aba65] Submitted process > NFCORE_RNASEQ:RNASEQ:MULTIQC (1)
-[nf-core/rnaseq] Pipeline completed successfully with skipped sampl(es)-
-[nf-core/rnaseq] Please check MultiQC report: 5/5 samples skipped since they failed 10000 trimmed read threshold.-
Nextflow stderr:

Nextflow 24.10.5 is available - Please consider updating your version to it