Error: Process requirement exceeds available memory

[Lara149]

Hi,

This is the Error I get:
ERROR ~ Error executing process > 'NFCORE_RNASEQ:RNASEQ:FASTQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE (WT2)'

Caused by:
Process requirement exceeds available memory -- req: 72 GB; avail: 15.6 GB

Is it somehow possible to implement the limited memory. I'm working on VM with 16 VPCUs and 16 GP RAM.

[davidecarlson]

You can use a custom config file to apply limits to how much memory is allowed to be used or how many CPUs are used.

Note however that depending on what species you are working with and what tools you are using, 16 GB of may not be sufficient to run the pipeline. For example, when I run the pipeline using a human reference genome, ~40 GB of memory is used by the STAR aligner.

Best,
Dave