@@ -17,93 +17,93 @@ disable_version_detection: true
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data_format : " yaml"
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run_modules :
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- - fastqc
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- - fastp
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- - adapterremoval
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- - custom_content
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- - bowtie2
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- - busco
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- - quast
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- - kraken
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- - prokka
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- - porechop
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- - filtlong
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- - trimmomatic
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+ - fastqc
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+ - fastp
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+ - adapterremoval
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+ - custom_content
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+ - bowtie2
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+ - busco
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+ - quast
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+ - kraken
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+ - prokka
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+ - porechop
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+ - filtlong
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+ - trimmomatic
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# # Module order
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top_modules :
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- - " fastqc " :
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- name : " FastQC: raw reads"
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- path_filters_exclude :
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- - " *trimmed*"
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- - " fastp"
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- - " adapterremoval"
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- - " trimmomatic"
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- - " porechop"
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- - " filtlong"
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- - " fastqc " :
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- name : " FastQC: after preprocessing"
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- info : " After trimming and, if requested, contamination removal."
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- path_filters :
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- - " *trimmed*"
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- - " bowtie2 " :
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- name : " Bowtie2: PhiX removal"
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- info : " Mapping statistics of reads mapped against PhiX and subsequently removed."
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- path_filters :
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- - " *_phix_removed.bowtie2.log"
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- - " bowtie2 " :
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- name : " Bowtie2: host removal"
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- info : " Mapping statistics of reads mapped against host genome and subsequently
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- removed."
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- path_filters :
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- - " *_host_removed.bowtie2.log"
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- - " kraken " :
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- name : " Kraken2"
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- anchor : " Kraken2"
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- target : " Kraken2"
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- doi : " 10.1101/gr.210641.116"
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- path_filters :
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- - " *.kraken2_report.txt"
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- - " kraken " :
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- name : " Centrifuge"
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- anchor : " centrifuge"
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- target : " Centrifuge"
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- doi : " 10.1101/gr.210641.116"
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- info : " is a very rapid and memory-efficient system for the classification of DNA
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- sequences from microbial samples. The system uses a novel indexing scheme based
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- on the Burrows-Wheeler transform (BWT) and the Ferragina-Manzini (FM) index.
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- Note: Figure title"
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- extra : " ℹ️: plot title will say Kraken2 due to Centrifuge producing the same output
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- format as Kraken. If activated, see the actual Kraken2 results in the section
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- above."
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- path_filters :
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- - " *.centrifuge_kreport.txt"
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- - " quast " :
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- name : " QUAST: assembly"
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- info : " Assembly statistics of raw assemblies."
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- path_filters :
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- - " *rawassemblies.tsv"
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- - " bowtie2 " :
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- name : " Bowtie2: assembly"
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- info : " Mapping statistics of reads mapped against assemblies."
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- path_filters_exclude :
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- - " *_host_removed.bowtie2.log"
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- - " *_phix_removed.bowtie2.log"
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- - " bcftools"
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- - custom_content
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- - " quast " :
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- name : " QUAST: bins"
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- info : " Assembly statistics of binned assemblies."
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- path_filters_exclude :
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- - " *rawassemblies.tsv"
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- - " busco " :
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- info : " assesses genome assembly and annotation completeness with Benchmarking
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- Universal Single-Copy Orthologs. In case BUSCO's automated lineage selection
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- was used, only generic results for the selected domain are shown and only for
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- genome bins and kept, unbinned contigs for which the BUSCO analysis was successfull,
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- i.e. not for contigs for which no BUSCO genes could be found. Bins for which
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- a specific virus lineage was selected are also not shown."
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- - " prokka"
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+ - " fastqc " :
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+ name : " FastQC: raw reads"
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+ path_filters_exclude :
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+ - " *trimmed*"
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+ - " fastp"
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+ - " adapterremoval"
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+ - " trimmomatic"
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+ - " porechop"
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+ - " filtlong"
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+ - " fastqc " :
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+ name : " FastQC: after preprocessing"
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+ info : " After trimming and, if requested, contamination removal."
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+ path_filters :
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+ - " *trimmed*"
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+ - " bowtie2 " :
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+ name : " Bowtie2: PhiX removal"
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+ info : " Mapping statistics of reads mapped against PhiX and subsequently removed."
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+ path_filters :
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+ - " *_phix_removed.bowtie2.log"
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+ - " bowtie2 " :
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+ name : " Bowtie2: host removal"
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+ info : " Mapping statistics of reads mapped against host genome and subsequently
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+ removed."
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+ path_filters :
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+ - " *_host_removed.bowtie2.log"
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+ - " kraken " :
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+ name : " Kraken2"
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+ anchor : " Kraken2"
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+ target : " Kraken2"
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+ doi : " 10.1101/gr.210641.116"
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+ path_filters :
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+ - " *.kraken2_report.txt"
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+ - " kraken " :
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+ name : " Centrifuge"
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+ anchor : " centrifuge"
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+ target : " Centrifuge"
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+ doi : " 10.1101/gr.210641.116"
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+ info : " is a very rapid and memory-efficient system for the classification of DNA
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+ sequences from microbial samples. The system uses a novel indexing scheme based
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+ on the Burrows-Wheeler transform (BWT) and the Ferragina-Manzini (FM) index.
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+ Note: Figure title"
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+ extra : " ℹ️: plot title will say Kraken2 due to Centrifuge producing the same output
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+ format as Kraken. If activated, see the actual Kraken2 results in the section
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+ above."
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+ path_filters :
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+ - " *.centrifuge_kreport.txt"
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+ - " quast " :
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+ name : " QUAST: assembly"
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+ info : " Assembly statistics of raw assemblies."
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+ path_filters :
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+ - " *rawassemblies.tsv"
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+ - " bowtie2 " :
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+ name : " Bowtie2: assembly"
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+ info : " Mapping statistics of reads mapped against assemblies."
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+ path_filters_exclude :
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+ - " *_host_removed.bowtie2.log"
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+ - " *_phix_removed.bowtie2.log"
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+ - " bcftools"
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+ - custom_content
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+ - " quast " :
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+ name : " QUAST: bins"
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+ info : " Assembly statistics of binned assemblies."
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+ path_filters_exclude :
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+ - " *rawassemblies.tsv"
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+ - " busco " :
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+ info : " assesses genome assembly and annotation completeness with Benchmarking
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+ Universal Single-Copy Orthologs. In case BUSCO's automated lineage selection
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+ was used, only generic results for the selected domain are shown and only for
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+ genome bins and kept, unbinned contigs for which the BUSCO analysis was successfull,
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+ i.e. not for contigs for which no BUSCO genes could be found. Bins for which
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+ a specific virus lineage was selected are also not shown."
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+ - " prokka"
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custom_data :
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host_removal :
@@ -133,14 +133,14 @@ sp:
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# # File name cleaning
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extra_fn_clean_exts :
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- - " .bowtie2"
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- - " _ar2"
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- - " host_removed"
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- - " phix_removed"
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- - " centrifuge_kreport"
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- - " _fastp"
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- - " _trimmomatic_trim.log"
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- - " _trimmomatic_trim"
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+ - " .bowtie2"
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+ - " _ar2"
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+ - " host_removed"
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+ - " phix_removed"
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+ - " centrifuge_kreport"
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+ - " _fastp"
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+ - " _trimmomatic_trim.log"
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+ - " _trimmomatic_trim"
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# # Prettification
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custom_logo : " mag_logo_mascot_light.png"
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