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| 1 | +<h1>Morphology Plot</h1> |
| 2 | +<p> |
| 3 | + This widget is a histogram based analysis tool which provides information on |
| 4 | + nodes with a similar distance to a reference location, which can be a specified |
| 5 | + in the user interface. Example choices are the root node or the average node |
| 6 | + position. Around this center, spheres are formed with an increasing radius that |
| 7 | + can be defined by the user and defaults to 1000nm per step. All nodes within the |
| 8 | + part of a sphere that doesn\'t overlap with the last smaller sphere is treated |
| 9 | + as one bin of a histogram. This is shown for three bins in 2D below:</p> |
| 10 | +<p> |
| 11 | + <svg height="100" with="100" viewBox="0 0 100 100" xmlns="http://www.w3.org/2000/svg"> |
| 12 | + <circle cx="50" cy="50" r="49" style="stroke: #000; fill: #fff"/> |
| 13 | + <circle cx="50" cy="50" r="30" style="stroke: #000; fill: #fff"/> |
| 14 | + <circle cx="50" cy="50" r="10" style="stroke: #000; fill: #fff"/> |
| 15 | + <text x="50" y="53" style="font-size: 10px; text-anchor: middle">1</text> |
| 16 | + <text x="31" y="53" style="font-size: 10px; text-anchor: middle">2</text> |
| 17 | + <text x="10" y="53" style="font-size: 10px; text-anchor: middle">3</text> |
| 18 | + </svg> |
| 19 | +</p> |
| 20 | +<p> |
| 21 | + Which nodes are part of which bin depends heavily on the morphology of a |
| 22 | + neuron. The widget allows then to compute various metrics on those bins: |
| 23 | + <ul> |
| 24 | + <li><b>Sholl analysis:</b> The number of edges intersecting with the boundary |
| 25 | + of the smaller sphere. Notice that if parent-child segments are longer than |
| 26 | + radius-increment in the radial direction, some parent-child segments will be |
| 27 | + counted more than once, which is correct.</li> |
| 28 | + <li><b>Radial density of cable:</b> Approximate aggregated cable length per |
| 29 | + bin by adding the length of all child-parent edges of all child nodes in the |
| 30 | + bin.</li> |
| 31 | + <li><b>Radial density of branch nodes:</b>The number of branch nodes in a |
| 32 | + particular bin.</li> |
| 33 | + <li><b>Radial density of ends:</b> The number of end nodes in a particular |
| 34 | + bin.</li> |
| 35 | + <li><b>Radial density of input synapses:</b> The number of input synapses in |
| 36 | + a particular bin.</li> |
| 37 | + <li><b>Radial density of output synapses:</b> The number of output synapses |
| 38 | + in a particular bin.</li> |
| 39 | + <li><b>Radial density of gap junctions:</b> The number of gap junctions in a |
| 40 | + particular bin.</li> |
| 41 | + <li><b>Radial density of desmosomes:</b> The number of desmosomes in a |
| 42 | + particular bin.</li> |
| 43 | + </ul> |
| 44 | +</p> |
| 45 | +<p> |
| 46 | + The plot itself shows the distance from the selected center on the X axis and |
| 47 | + the bin value (e.g. the number of end nodes) is shown on the Y axis. |
| 48 | +<p> |
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