diff --git a/packages/vite/src/node/plugins/css.ts b/packages/vite/src/node/plugins/css.ts
index 378a2a193eb2f8..f3c4d1ec512775 100644
--- a/packages/vite/src/node/plugins/css.ts
+++ b/packages/vite/src/node/plugins/css.ts
@@ -1207,6 +1207,8 @@ export function getEmptyChunkReplacer(
     )
 }
 
+const fileURLWithWindowsDriveRE = /^file:\/\/\/[a-zA-Z]:\//
+
 interface CSSAtImportResolvers {
   css: ResolveIdFn
   sass: ResolveIdFn
@@ -1245,7 +1247,9 @@ function createCSSResolvers(config: ResolvedConfig): CSSAtImportResolvers {
             args[1] = fileURLToPath(args[1], {
               windows:
                 // file:///foo cannot be converted to path with windows mode
-                isWindows && args[1].startsWith('file:///') ? false : undefined,
+                isWindows && !fileURLWithWindowsDriveRE.test(args[1])
+                  ? false
+                  : undefined,
             })
           }
           return resolver(...args)